Labelling and immunoprecipitation of thyroid microsomal antigen

[Studies on rat sera immunized with solubilized thyroid microsomal antigen].

Thyroid microsomal antigen was studied using sera from rats immunized with solubilized human thyroid microsomal fraction. The methods used were gel immunodiffusion and the fluorescent antibody technique (FAT). Kidney, liver and thyroid microsomal fractions were solubilized by 0.5% Triton X-100 PBS. Rat sera immunized with solubilized thyroid microsomal fraction had a few precipitin antibodies t...

Characterization of the thyroid microsomal antigen, and its relationship to thyroid peroxidase, using monoclonal antibodies.

MAb directed to the thyroid microsomal antigen have been developed. All bound to 101- and 107-kD bands in Western blot analysis using thyroid microsomal fraction as antigen. The MAb also bound to microsomal proteins immunoprecipitated by serum from patients having a high titer of anti-microsomal antibody but no antibodies to thyroglobulin or thyrotropin-stimulating hormone receptor. The pattern...

Antigen-antibody reactions Immunoagglutination Immunoprecipitation

[Anti-thyroglobulin and anti-thyroid microsomal antibodies in thyroid disorders].

Serum anti-thyroglobulin antibody (TgAb) and anti-thyroid microsomal antibody (McAb) in 213 cases of thyroid disorders and 32 cases of non-thyroid disorders were studied. The antibodies were checked by commercial tanned red cell hemagglutination kits (Thymune-T and Thymune-M) purchased from Wellcome Reagents, Limited, England. The positive rates of TgAb and McAb in patients with untreated toxic...

Isolation of a complementary DNA clone for thyroid microsomal antigen. Homology with the gene for thyroid peroxidase.

The thyroid microsomal antigen (MSA) in autoimmune thyroid disease is a protein of approximately 107 kD. We screened a human thyroid cDNA library constructed in the expression vector lambda gt11 with anti-107-kD monoclonal antibodies. Of five clones obtained, the recombinant beta-galactosidase fusion protein from one clone (PM-5) was confirmed to react with the monoclonal antiserum. The complem...